Journal: Cell reports

Article Title: Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis

doi: 10.1016/j.celrep.2020.02.040

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: After deparaffinization, heat-induced antigen retrieval and blocking, sections were stained with 5 μg/ml Polyclonal Goat Anti-Mouse CCL11 antibody (R&D Systems) and Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems) and counter stained with Hematoxylin.

Techniques: Recombinant, Staining, cDNA Synthesis, SYBR Green Assay, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Software

Journal: Bone & Joint Research

Article Title: The promising regenerative potential of pulsed electromagnetic fields toward tendon differentiation

doi: 10.1302/2046-3758.152.BJR-2024-0550.R2

Figure Lengend Snippet: Scleraxis expression and fluorescence quantification in hamstring tendon explant sections with or without exposure to pulsed electromagnetic fields (PEMF) for 21 days, 8 hr/day. Representative images are shown. Left: representative immunostaining images for b) no PEMF and d) PEMF groups and their negative controls (a and c). Image d shows increased fluorescence intensity compared to image b. Yellow arrows in the images indicate representative localization of the specific markers. Scale bar: 25 μm. Right: graph depicting the results for each marker including the statistical analysis. Results are shown as mean (standard error of the mean) of corrected total cell fluorescence (CTCF). *Statistically significant p-value (< 0.05) in a two-tailed independent-samples t -test. Nine samples were evaluated.

Article Snippet: Immunohistochemistry stainings were performed using the Caspase-3 Cleaved (Asp175) antibody #9661 (Cell Signaling Technology, USA) with the avidin-biotin-peroxidase techniques (Anti-Mouse HRP-DAB Cell & Tissue Staining Kit, R&D Systems, USA).

Techniques: Expressing, Fluorescence, Immunostaining, Marker, Two Tailed Test

Journal: Bone & Joint Research

Article Title: The promising regenerative potential of pulsed electromagnetic fields toward tendon differentiation

doi: 10.1302/2046-3758.152.BJR-2024-0550.R2

Figure Lengend Snippet: c-Fos expression and fluorescence quantification in hamstring tendon explant sections with or without exposure to pulsed electromagnetic fields (PEMF) for 21 days, 8 hr/day. Representative images are shown. Left: representative immunostaining images for b) no PEMF and d) PEMF groups and their negative controls (a and c). Image d shows increased fluorescence intensity compared to image b. Yellow arrows in the images indicate representative localization of the specific markers. Scale bar: 25 μm. Right: graph depicting the results for each marker including the statistical analysis. Results are shown as mean (standard error of the mean) of corrected total cell fluorescence (CTCF). *Statistically significant p-value (< 0.05) in a two-tailed independent-samples t -test. Nine samples were evaluated.

Article Snippet: Immunohistochemistry stainings were performed using the Caspase-3 Cleaved (Asp175) antibody #9661 (Cell Signaling Technology, USA) with the avidin-biotin-peroxidase techniques (Anti-Mouse HRP-DAB Cell & Tissue Staining Kit, R&D Systems, USA).

Techniques: Expressing, Fluorescence, Immunostaining, Marker, Two Tailed Test

Journal: Bone & Joint Research

Article Title: The promising regenerative potential of pulsed electromagnetic fields toward tendon differentiation

doi: 10.1302/2046-3758.152.BJR-2024-0550.R2

Figure Lengend Snippet: Mammalian target of rapamycin (mTOR) expression and fluorescence quantification in hamstring tendon explant sections with or without exposure to pulsed electromagnetic fields (PEMF) for 21 days, 8 hr/day. Representative images are shown. Left: representative immunostaining images for b) no PEMF and d) PEMF groups and their negative controls (a and c). Image d shows increased fluorescence intensity compared to image b. Yellow arrows in the images indicate representative localization of the specific markers. Scale bar: 25 μm. Right: graph depicting the results for each marker including the statistical analysis. Results are shown as mean (standard error of the mean) of corrected total cell fluorescence (CTCF). *Statistically significant p-value (< 0.05) in a two-tailed independent-samples t -test. Nine samples were evaluated.

Article Snippet: Immunohistochemistry stainings were performed using the Caspase-3 Cleaved (Asp175) antibody #9661 (Cell Signaling Technology, USA) with the avidin-biotin-peroxidase techniques (Anti-Mouse HRP-DAB Cell & Tissue Staining Kit, R&D Systems, USA).

Techniques: Expressing, Fluorescence, Immunostaining, Marker, Two Tailed Test

Journal: Bone & Joint Research

Article Title: The promising regenerative potential of pulsed electromagnetic fields toward tendon differentiation

doi: 10.1302/2046-3758.152.BJR-2024-0550.R2

Figure Lengend Snippet: Proliferating cell nuclear antigen (PCNA) expression and fluorescence quantification in hamstring tendon explant sections with or without exposure to pulsed electromagnetic fields (PEMF) for 21 days, 8 hr/day. Representative images are shown. Left: representative immunostaining images for b) no PEMF and d) PEMF groups and their negative controls (a and c). No significant difference was observed in the number of PCNA-positive cells between the two groups. Scale bar: 25 μm. Right: graph depicting the results for each marker including the statistical analysis. Results are shown as mean (standard error of the mean) of the ratio between the number of PCNA-positive cells and the total number of cells in each field. Nine samples were evaluated. ns, non-significant.

Article Snippet: Immunohistochemistry stainings were performed using the Caspase-3 Cleaved (Asp175) antibody #9661 (Cell Signaling Technology, USA) with the avidin-biotin-peroxidase techniques (Anti-Mouse HRP-DAB Cell & Tissue Staining Kit, R&D Systems, USA).

Techniques: Expressing, Fluorescence, Immunostaining, Marker

Journal: Bone & Joint Research

Article Title: The promising regenerative potential of pulsed electromagnetic fields toward tendon differentiation

doi: 10.1302/2046-3758.152.BJR-2024-0550.R2

Figure Lengend Snippet: β-catenin expression and fluorescence quantification in hamstring tendon explant sections with or without exposure to pulsed electromagnetic fields (PEMF) for 21 days, 8 hr/day. Representative images are shown. Left: representative immunostaining images for b) no PEMF and d) PEMF groups and their negative controls (a and c). No significant difference was observed between the two groups. Image d is representative of the tendency toward decreased β-catenin signal. Yellow arrows in the images indicate representative localization of the specific markers. Scale bar: 25 μm. Right: graph depicting the results for each marker including the statistical analysis. Results are shown as mean (standard error of the mean) of corrected total cell fluorescence (CTCF). Nine samples were evaluated. ns, non-significant.

Article Snippet: Immunohistochemistry stainings were performed using the Caspase-3 Cleaved (Asp175) antibody #9661 (Cell Signaling Technology, USA) with the avidin-biotin-peroxidase techniques (Anti-Mouse HRP-DAB Cell & Tissue Staining Kit, R&D Systems, USA).

Techniques: Expressing, Fluorescence, Immunostaining, Marker

Journal: Bone & Joint Research

Article Title: The promising regenerative potential of pulsed electromagnetic fields toward tendon differentiation

doi: 10.1302/2046-3758.152.BJR-2024-0550.R2

Figure Lengend Snippet: Relative messenger RNA (mRNA) levels of a) scleraxis, b) collagen type I, c) collagen type VI, d) c-Fos, e) mammalian target of rapamycin (mTOR), f) proliferating cell nuclear antigen (PCNA), and g) β-catenin in tendon explants exposed to pulsed electromagnetic fields (PEMF) for 21 days, 8 hr/day, compared to tendon explants not exposed to PEMF measured by quantitative reverse transcription polymerase chain reaction analysis. Results were calculated as fold changes (mean (SD) of triplicates) relative to controls, normalized on 18S RNA level. *Statistically significant p-value (p < 0.05) in a two-tailed independent-samples t -test; ***Statistically significant p-value (p < 0.001) in a two-tailed independent-samples t -test. Three samples were evaluated. ns, non-significant.

Article Snippet: Immunohistochemistry stainings were performed using the Caspase-3 Cleaved (Asp175) antibody #9661 (Cell Signaling Technology, USA) with the avidin-biotin-peroxidase techniques (Anti-Mouse HRP-DAB Cell & Tissue Staining Kit, R&D Systems, USA).

Techniques: Reverse Transcription, Polymerase Chain Reaction, Two Tailed Test