Journal: Cell reports

Article Title: Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis

doi: 10.1016/j.celrep.2020.02.040

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: After deparaffinization, heat-induced antigen retrieval and blocking, sections were stained with 5 μg/ml Polyclonal Goat Anti-Mouse CCL11 antibody (R&D Systems) and Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems) and counter stained with Hematoxylin.

Techniques: Recombinant, Staining, cDNA Synthesis, SYBR Green Assay, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Software

Journal: International Journal of Molecular Sciences

Article Title: GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

doi: 10.3390/ijms23062913

Figure Lengend Snippet: Determination of H 2 O 2 content and activities at 2 dpi. ( a ) Detection of ROS levels in cotton leaves by H 2 DCFDA staining. Then 10 mM H 2 DCFDA ROS—specific fluorescent indicator was used for the staining. Bars = 100 um. ( b ) H 2 O 2 visualization in cotton leaves by staining with DAB, showing more H 2 O 2 was produced in the TRV . 00 than the silent plants. Bars = 200 um. ( c – f ) Measurements of the contents of H 2 O 2 and SOD in cotton and Arabidopsis leaves at 2 dpi. Values are means with SD ( n = 3 biological replicates). Error bars represent the SD of three biological replicates. Lowercase letters represent statistically significant differences ( p < 0.05) according to Tukey’s HSD test.

Article Snippet: In order to observe the accumulation of H 2 O 2 , using the plant hydrogen peroxide DAB staining kit (servicebio), fresh cotton leaves were taken, incubated in 1 mg/mL, pH 3.8 DAB solution for 8 h, and then decolorized in 95% ethanol [ ].

Techniques: Staining, Produced